I applied my second application of nematodes on Tues. Apr. 4th. I had some new ant mounds that had popped up from earlier rains. Instead of applying it in close proximity  to the mounds, as I had done previously, I used a hos- end sprayer to broadcast the nematodes on a broader area. After my fist application I was seeing little to no activity in any of the mounds within a few day. This time after applying on the whole yard instead of spot application, the results took over a week, but there is again little to no activity with the ant mounds. I am hoping by applying it on the whole yard that it will have effect all year long with the fire ants.... to be continued.